A novel signal sequence negative multimeric glycosomal protein required for cell cycle progression of Leishmania donovani parasites.

A novel signal sequence negative multimeric glycosomal protein required for cell cycle progression of Leishmania donovani parasites.

Expression of the intracellular type amastigote particular genes within the Leishmania donovani parasite performs a serious position in parasite replication within the macrophage. Within the present work, we’ve characterised a novel hypothetical gene, Ld30b that’s particularly transcribed within the intracellular stage of the parasite. The recombinant Ld30b protein exists as a pentamer in answer as recognized by native-PAGE and measurement exclusion gel chromatography.

Structural evaluation utilizing round dichroism and molecular modeling point out that Ld30b belongs to household of cAMP-dependent protein kinase sort I-alpha regulatory subunit. Co-localization immunofluorescence microscopy and western blot analyses (utilizing anti-Ld30b antibody and anti-hypoxanthine-guanine phosphoribosyl transferase, a glycosome marker) on the remoted parasite glycosome organelle fractions present that Ld30b is localized in glycosome, although lacked a glycosome focusing on PTS1/2 sign within the protein sequence.

Episomal expression of Ld30b within the parasite precipitated the arrest of promastigotes and amastigotes progress in vitro. Cell cycle evaluation utilizing movement cytometry signifies that these parasites are arrested in ‘sub G0/G1’ section of the cell cycle. Single allele knockout of Ld30b within the parasite equally attenuated its progress by accumulation of cells within the S section of cell cycle, thus confirming the possible significance of acceptable stage of protein within the cells. Finding out such intracellular stage expressing genes may unravel novel regulatory pathways for the event of medicine or vaccine candidates towards leishmaniasis.

A big-scale expression technique for multimeric extracellular protein complexes utilizing Drosophila S2 cells and its software to the recombinant expression of heterodimeric ligand-binding domains of style receptor.

Most of the extracellular proteins or extracellular domains of plasma membrane proteins exist or operate as homo- or heteromeric multimer protein complexes. Profitable recombinant manufacturing of such proteins is usually achieved by co-expression of the parts utilizing eukaryotic cells by way of the secretory pathway. Right here we report a method addressing large-scale expression of hetero-multimeric extracellular domains of plasma membrane proteins and its software to the extracellular domains of a style receptor.

The goal receptor consists of a heterodimer of T1r2 and T1r3 proteins, and their extracellular ligand binding domains (LBDs) are accountable for the notion of main style substances. Nonetheless, regardless of the useful significance, recombinant manufacturing of the heterodimeric proteins has to this point been unsuccessful. We achieved the profitable preparation of the heterodimeric LBD by use of Drosophila S2 cells, which have a excessive secretory capability, and by the institution of a secure high-expression clone producing each subunits at a comparable stage.

The strategy overcame the issues encountered within the typical transient expression of the receptor protein in insect cells utilizing baculovirus or vector lipofection, which failed within the correct heterodimer manufacturing due to the biased expression of T1r3LBD over T1r2LBD. The big-scale expression methodology reported right here might function one of many appreciable methods for the preparation of multimeric extracellular protein complexes.

Fourier Remodel-Ion Cyclotron Resonance Mass Spectrometry as a Platform for Characterizing Multimeric Membrane Protein Complexes.

Membrane protein characterization is constantly hampered by challenges with expression, purification, and solubilization. Amongst a number of biophysical strategies employed for his or her characterization, native-mass spectrometry (MS) has emerged as a strong software for the evaluation of membrane proteins and complexes. Right here, two MS platforms, the FT-ICR and Q-ToF, have been explored to research the homotetrameric water channel protein, AquaporinZ (AqpZ), beneath non-denaturing circumstances.

This 97 kDa membrane protein advanced will be readily liberated from the octylglucoside (OG) detergent micelle beneath a variety of instrument circumstances on each MS platforms. Growing the utilized collision power of the FT-ICR collision cell yielded various levels of tetramer (97 kDa) liberation from the OG micelles, in addition to dissociation into the trimeric (72 kDa) and monomeric (24 kDa) substituents.

A novel signal sequence negative multimeric glycosomal protein required for cell cycle progression of Leishmania donovani parasites.

Tandem-MS on the Q-ToF yielded increased depth tetramer sign and, relying on the m/z area chosen, the noticed monomer sign assorted in depth. Precursor ion collection of an m/z vary above the anticipated protein sign distribution, adopted by gentle collisional activation, is ready to effectively liberate AqpZ with a excessive S/N ratio. The tetrameric cost state distribution obtained on each devices demonstrated superpositioning of a number of proteoforms because of various levels of N-terminal formylation. Graphical Summary ᅟ

The most important outer sheath protein kinds distinct conformers and multimeric complexes within the outer membrane and periplasm of Treponema denticola.

The most important outer sheath protein (MOSP) is a outstanding constituent of the cell envelope of Treponema denticola (TDE) and one in every of its principal virulence determinants. Bioinformatics predicts that MOSP consists of N- and C-terminal domains, MOSPN and MOSPC. Biophysical evaluation of constructs refolded in vitro demonstrated that MOSPC, beforehand proven to own porin exercise, kinds amphiphilic trimers, whereas MOSPN kinds an prolonged hydrophilic monomer.

In TDE and E. coli expressing MOSP with a PelB sign sequence (PelB-MOSP), MOSPC is OM-embedded and surface-exposed, whereas MOSPN resides within the periplasm. Immunofluorescence assay, floor proteolysis, and novel cell fractionation schemes revealed that MOSP in TDE exists as outer membrane (OM) and periplasmic trimeric conformers; PelB-MOSP, in distinction, fashioned solely OM-MOSP trimers.

Though each conformers type hetero-oligomeric complexes in TDE, solely OM-MOSP associates with dentilisin. Mass spectrometry (MS) indicated that OM-MOSP interacts with proteins along with dentilisin, most notably, oligopeptide-binding proteins (OBPs) and the β-barrel of BamA. MS additionally recognized candidate companions for periplasmic MOSP, together with TDE1658, a spirochete-specific SurA/PrsA ortholog.

Human NADH Dehydrogenase, Quinone 1 (NQO1) ELISA Kit

DLR-NQO1-Hu-96T 96T
EUR 725
Description: A sandwich quantitative ELISA assay kit for detection of Human NADH Dehydrogenase, Quinone 1 (NQO1) in samples from tissue homogenates, cell lysates or other biological fluids.

Anti-NQO1 Antibody

PB9497 100ug/vial
EUR 294

Anti-NQO1 antibody

STJ24804 100 µl
EUR 277
Description: This gene is a member of the NAD(P)H dehydrogenase (quinone) family and encodes a cytoplasmic 2-electron reductase. This FAD-binding protein forms homodimers and reduces quinones to hydroquinones. This protein's enzymatic activity prevents the one electron reduction of quinones that results in the production of radical species. Mutations in this gene have been associated with tardive dyskinesia (TD), an increased risk of hematotoxicity after exposure to benzene, and susceptibility to various forms of cancer. Altered expression of this protein has been seen in many tumors and is also associated with Alzheimer's disease (AD). Alternate transcriptional splice variants, encoding different isoforms, have been characterized.

Anti-NQO1 antibody

STJ24805 100 µl
EUR 277
Description: This gene is a member of the NAD(P)H dehydrogenase (quinone) family and encodes a cytoplasmic 2-electron reductase. This FAD-binding protein forms homodimers and reduces quinones to hydroquinones. This protein's enzymatic activity prevents the one electron reduction of quinones that results in the production of radical species. Mutations in this gene have been associated with tardive dyskinesia (TD), an increased risk of hematotoxicity after exposure to benzene, and susceptibility to various forms of cancer. Altered expression of this protein has been seen in many tumors and is also associated with Alzheimer's disease (AD). Alternate transcriptional splice variants, encoding different isoforms, have been characterized.

Anti-NQO1 antibody

STJ94551 200 µl
EUR 197
Description: Rabbit polyclonal to NQO1.

Anti-NQO1 antibody

STJ70143 100 µg
EUR 359

anti- NQO1 antibody

FNab05830 100µg
EUR 505.25
Description: Antibody raised against NQO1

anti-NQO1 (4D12)

LF-MA30655 100 ul
EUR 527
Description: Mouse Monoclonal to NQO1

anti-NQO1 (1A11)

LF-MA30656 100 ul
EUR 527
Description: Mouse Monoclonal to NQO1

Anti-NQO1 antibody

PAab05830 100 ug
EUR 355

Mouse NADH Dehydrogenase, Quinone 1 (NQO1) ELISA Kit

RDR-NQO1-Mu-48Tests 48 Tests
EUR 603

Mouse NADH Dehydrogenase, Quinone 1 (NQO1) ELISA Kit

RDR-NQO1-Mu-96Tests 96 Tests
EUR 840

Rat NADH Dehydrogenase, Quinone 1 (NQO1) ELISA Kit

RDR-NQO1-Ra-48Tests 48 Tests
EUR 631

Rat NADH Dehydrogenase, Quinone 1 (NQO1) ELISA Kit

RDR-NQO1-Ra-96Tests 96 Tests
EUR 880

Mouse NADH Dehydrogenase, Quinone 1 (NQO1) ELISA Kit

RD-NQO1-Mu-48Tests 48 Tests
EUR 577

Mouse NADH Dehydrogenase, Quinone 1 (NQO1) ELISA Kit

RD-NQO1-Mu-96Tests 96 Tests
EUR 802

Rat NADH Dehydrogenase, Quinone 1 (NQO1) ELISA Kit

RD-NQO1-Ra-48Tests 48 Tests
EUR 603

Rat NADH Dehydrogenase, Quinone 1 (NQO1) ELISA Kit

RD-NQO1-Ra-96Tests 96 Tests
EUR 840

Mouse NADH Dehydrogenase, Quinone 1 (NQO1) ELISA Kit

DLR-NQO1-Mu-48T 48T
EUR 566
Description: A sandwich quantitative ELISA assay kit for detection of Mouse NADH Dehydrogenase, Quinone 1 (NQO1) in samples from tissue homogenates, cell lysates or other biological fluids.

Mouse NADH Dehydrogenase, Quinone 1 (NQO1) ELISA Kit

DLR-NQO1-Mu-96T 96T
EUR 741
Description: A sandwich quantitative ELISA assay kit for detection of Mouse NADH Dehydrogenase, Quinone 1 (NQO1) in samples from tissue homogenates, cell lysates or other biological fluids.

Rat NADH Dehydrogenase, Quinone 1 (NQO1) ELISA Kit

DLR-NQO1-Ra-48T 48T
EUR 590
Description: A sandwich quantitative ELISA assay kit for detection of Rat NADH Dehydrogenase, Quinone 1 (NQO1) in samples from tissue homogenates, cell lysates or other biological fluids.

Rat NADH Dehydrogenase, Quinone 1 (NQO1) ELISA Kit

DLR-NQO1-Ra-96T 96T
EUR 774
Description: A sandwich quantitative ELISA assay kit for detection of Rat NADH Dehydrogenase, Quinone 1 (NQO1) in samples from tissue homogenates, cell lysates or other biological fluids.

Rabbit Polyclonal antibody Anti-CRBN

Anti-CRBN 50 µg
EUR 349

Anti-NQO1 (1E3-A6)

YF-MA12677 100 ug
EUR 363
Description: Mouse monoclonal to NQO1

NQO1 Recombinant Protein (Human)

RP078738 100 ug Ask for price

Anti-NQO1 Monoclonal Antibody (A180)

M00494-1 200ug
EUR 498
Description: Mouse Monoclonal NQO1 Antibody (A180). Validated in WB and tested in Human.

Anti-NQO1 (isoform a) antibody

STJ72517 100 µg
EUR 359

Polyclonal Goat Anti-NQO1 Antibody

AMM05061G 0.1 mg
EUR 484
Description: A polyclonal antibody raised in Goat that recognizes and binds to Human Goat Anti-NQO1 . This antibody is tested and proven to work in the following applications:

Nqo1/ Rat Nqo1 ELISA Kit

ELI-06921r 96 Tests
EUR 886

Human NQO1 Antibody

32959-05111 150 ug
EUR 261

NQO1, human recombinant

7571-10
EUR 327

NQO1, human recombinant

7571-50
EUR 936

h NQO1

LVP1058 1x107 IFU/ml x 200ul
EUR 451
Description: Pre-made over-expression lentivirus for expressing human target: NQO1 (NAD(P)H quinone dehydrogenase 1), [alternative names: DHQU; DIA4; DTD; NMOR1; NMORI; QR1]. The sub-cloned codon sequence is identical (100% match) to CDS region in NCBI ID: NM_000903. It also contains a RFP-Blasticidin dual selection marker.

NQO1 siRNA

20-abx926309
  • EUR 551.00
  • EUR 732.00
  • 15 nmol
  • 30 nmol

NQO1 siRNA

20-abx926310
  • EUR 551.00
  • EUR 732.00
  • 15 nmol
  • 30 nmol

NQO1 antibody

22937-100ul 100ul
EUR 390

NQO1 Antibody

31283-100ul 100ul
EUR 252

NQO1 Antibody

31283-50ul 50ul
EUR 187

NQO1 Antibody

35365-100ul 100ul
EUR 390

NQO1 Antibody

32295-100ul 100ul
EUR 252

NQO1 siRNA

20-abx903636
  • EUR 551.00
  • EUR 732.00
  • 15 nmol
  • 30 nmol

NQO1 Antibody

1-CSB-PA102829
  • EUR 317.00
  • EUR 244.00
  • 100ul
  • 50ul
Description: A polyclonal antibody against NQO1. Recognizes NQO1 from Human, Mouse, Rat. This antibody is Unconjugated. Tested in the following application: ELISA, WB, IHC;ELISA:1:1000-1:5000, WB:1:500-1:2000, IHC:1:25-1:100

NQO1 Antibody

1-CSB-PA016039GA01HU
  • EUR 597.00
  • EUR 333.00
  • 150ul
  • 50ul
Description: A polyclonal antibody against NQO1. Recognizes NQO1 from Human, Mouse, Rat. This antibody is Unconjugated. Tested in the following application: ELISA, WB, IHC, IF

NQO1 Antibody

1-CSB-PA016039LA01HU
  • EUR 317.00
  • EUR 335.00
  • 100ug
  • 50ug
Description: A polyclonal antibody against NQO1. Recognizes NQO1 from Human. This antibody is Unconjugated. Tested in the following application: ELISA, IHC, IF; Recommended dilution: IHC:1:20-1:200, IF:1:50-1:200

NQO1 Antibody

1-CSB-PA890070
  • EUR 317.00
  • EUR 244.00
  • 100ul
  • 50ul
Description: A polyclonal antibody against NQO1. Recognizes NQO1 from Human, Mouse, Rat. This antibody is Unconjugated. Tested in the following application: ELISA, WB, IHC;ELISA:1:2000-1:10000, WB:1:500-1:2000, IHC:1:50-1:200

NQO1 Antibody

1-CSB-PA003475
  • EUR 222.00
  • EUR 195.00
  • 100ug
  • 50ug
Description: A polyclonal antibody against NQO1. Recognizes NQO1 from Human. This antibody is Unconjugated. Tested in the following application: WB, ELISA;WB:1/500-1/2000.ELISA:1/10000

NQO1 Antibody

49364-100ul 100ul
EUR 333

NQO1 Antibody

49364-50ul 50ul
EUR 239

NQO1 Antibody

DF6437 200ul
EUR 304
Description: NQO1 Antibody detects endogenous levels of total NQO1.

NQO1 Antibody

ABD6437 100 ug
EUR 438

NQO1 Antibody

3639-100
EUR 349

NQO1 Antibody

3639-30T
EUR 146

pOTB7-NQO1

PVT14601 2 ug
EUR 495

NQO1 antibody

70R-18946 50 ul
EUR 435
Description: Rabbit polyclonal NQO1 antibody

NQO1 antibody

70R-10256 50 ug
EUR 467
Description: Affinity purified rabbit polyclonal NQO1 antibody

NQO1 antibody

70R-10257 50 ug
EUR 467
Description: Affinity purified rabbit polyclonal NQO1 antibody

NQO1 antibody

70R-12504 100 ul
EUR 457
Description: Affinity purified Rabbit polyclonal NQO1 antibody

NQO1 antibody

70R-49661 100 ul
EUR 244
Description: Purified Polyclonal NQO1 antibody

NQO1 Peptide

45-981P 0.1 mg
EUR 338
Description: NQO1 Peptide

NQO1 Peptide

42-451P 0.1 mg
EUR 338
Description: (IN) NQO1 Peptide

Polyclonal Goat anti-GST α-form

GST-ANTI-1 50 uL
EUR 280

Polyclonal Goat anti-GST μ-form

GST-ANTI-2 50 uL
EUR 280

Polyclonal Goat anti-GST p-form

GST-ANTI-3 50 uL
EUR 280

NQO1 Recombinant Protein (Mouse)

RP154820 100 ug Ask for price

NQO1 Recombinant Protein (Rat)

RP214418 100 ug Ask for price

NQO1 protein (His tag)

80R-1197 100 ug
EUR 586
Description: Purified recombinant Human NQO1 protein

Human NQO1 shRNA Plasmid

20-abx951197
  • EUR 801.00
  • EUR 1121.00
  • 150 µg
  • 300 µg

Human NQO1 ELISA Kit

ELA-E2154h 96 Tests
EUR 824

NQO1 ELISA KIT|Human

EF006205 96 Tests
EUR 689

Anti-NQO1/Dt Diaphorase Rabbit Monoclonal Antibody

M00494 100ug/vial
EUR 397
Description: Rabbit Monoclonal NQO1/Dt Diaphorase Antibody. Validated in IP, IF, WB and tested in Human, Mouse, Rat.

NQO1 activator 1

HY-128895 100mg
EUR 2633

NQO1 Polyclonal Antibody

ES2983-100ul 100ul
EUR 279
Description: A Rabbit Polyclonal antibody against NQO1 from Human. This antibody is tested and validated for WB, ELISA, WB, ELISA

NQO1 Polyclonal Antibody

ES2983-50ul 50ul
EUR 207
Description: A Rabbit Polyclonal antibody against NQO1 from Human. This antibody is tested and validated for WB, ELISA, WB, ELISA

NQO1 Polyclonal Antibody

ABP51984-003ml 0.03ml
EUR 158
Description: A polyclonal antibody for detection of NQO1 from Human. This NQO1 antibody is for WB, ELISA. It is affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthesized peptide derived from the C-terminal region of human NQO1

NQO1 Polyclonal Antibody

ABP51984-01ml 0.1ml
EUR 289
Description: A polyclonal antibody for detection of NQO1 from Human. This NQO1 antibody is for WB, ELISA. It is affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthesized peptide derived from the C-terminal region of human NQO1

NQO1 Polyclonal Antibody

ABP51984-02ml 0.2ml
EUR 414
Description: A polyclonal antibody for detection of NQO1 from Human. This NQO1 antibody is for WB, ELISA. It is affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthesized peptide derived from the C-terminal region of human NQO1

NQO1 Blocking Peptide

20-abx062536
  • EUR 272.00
  • EUR 411.00
  • 1 mg
  • 5 mg

NQO1 Rabbit pAb

A0047-100ul 100 ul
EUR 308

NQO1 Rabbit pAb

A0047-200ul 200 ul
EUR 459

NQO1 Rabbit pAb

A0047-20ul 20 ul
EUR 183

NQO1 Rabbit pAb

A0047-50ul 50 ul
EUR 223

NQO1 Rabbit pAb

A1518-100ul 100 ul
EUR 308

NQO1 Rabbit pAb

A1518-200ul 200 ul
EUR 459

NQO1 Rabbit pAb

A1518-20ul 20 ul
EUR 183

NQO1 Rabbit pAb

A1518-50ul 50 ul
EUR 223

NQO1 Rabbit mAb

A19586-100ul 100 ul
EUR 410

NQO1 Rabbit mAb

A19586-200ul 200 ul
EUR 571

NQO1 Rabbit mAb

A19586-20ul 20 ul
EUR 221

NQO1 Rabbit mAb

A19586-50ul 50 ul
EUR 287

Polyclonal NQO1 Antibody

APR08804G 0.1mg
EUR 484
Description: A polyclonal antibody raised in Rabbit that recognizes and binds to Human NQO1 . This antibody is tested and proven to work in the following applications:

NQO1 Polyclonal Antibody

41254-100ul 100ul
EUR 252

NQO1 Polyclonal Antibody

41254-50ul 50ul
EUR 187

Collectively, our knowledge counsel that MOSP destined for the TDE OM follows the canonical BAM pathway, whereas formation of a secure periplasmic conformer includes an export-related, folding pathway not current in E. coli.

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