Fragment Screening for 14 Different Biologically Active RNAs and 10 DNA and protein counter-screens

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19F-NMR-based fragment screening for 14 completely different biologically lively RNAs and 10 DNA and protein counter-screens

 

We report right here on the nuclear magnetic resonance (NMR) 19 F screening of 14 RNA targets with completely different secondary and tertiary construction to systematically assess druggability of RNAs. Our RNA targets embody consultant bacterial riboswitches that naturally bind with nanomolar affinity and excessive specificity to mobile metabolites of low molecular weight.
Primarily based on counter-screens in opposition to 5 DNAs and 5 proteins, we are able to present that RNA will be particularly focused. To reveal the standard of the preliminary fragment library that has been designed for simple follow-up chemistry, we additional present the best way to enhance binding affinity from an preliminary fragment hit by chemistry that hyperlinks the recognized fragment to the intercalator acridine. Thus, we obtain low micromolar binding affinity with out dropping binding specificity between two completely different terminator buildings.

Predicting persistence of atopic dermatitis in youngsters utilizing scientific attributes and serum proteins

 

Background: Atopic dermatitis (AD) is the commonest inflammatory pores and skin illness in youngsters, with 30% of all these identified creating power or relapsing illness by adolescence. Such illness persistence can’t but be predicted. The intention of the current research was to foretell the pure course of AD utilizing scientific parameters and serum proteins.
Strategies: Sera of 144 youngsters with AD (age 0-Three years) had been analyzed for IgE and 33 cytokines, chemokines, and development components. Affected person illness course till the age of seven years was assessed retrospectively. Unsupervised k-means clustering was carried out to outline illness endotypes.
Recognized components related to AD persistence on the age of seven years had been validated in youngsters with AD in an unbiased cohort (LISA Munich; n=168). Logistic regression and XGBoosting strategies adopted by cross-validation had been utilized to foretell particular person illness outcomes.
Outcomes: Three distinct endotypes had been present in infancy, characterised by a novel inflammatory signature. Elements related to illness persistence had been illness rating (SCORAD), involvement of the limbs, flexural lesion distribution on the age of three years, allergic comorbidities and illness exacerbation by the set off components stress, pollen publicity, and alter in climate.
Persistence was predicted with a sensitivity of 81.8% and a specificity of 82.4%. Elements with a excessive impression on the prediction of persistence had been SCORAD on the age of three years, set off components, and low VEGF serum ranges.
Conclusions: AD in infancy contains three immunological endotypes. Illness persistence will be predicted utilizing serum cytokines and scientific variables.
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LCK, Active Human Recombinant Protein

GWB-CBBFF1 10ug Ask for price

ITK, Active Human Recombinant Protein

GWB-BE69DC 10ug Ask for price

BTK, Active Human Recombinant Protein

GWB-6B7FE7 10ug Ask for price

Ret, Active Human Recombinant Protein

GWB-1E9110 10ug Ask for price

Bas, Active Human Recombinant Protein

GWB-PS58BD 20ug Ask for price

FES, Active Human Recombinant Protein

GWB-PS9783 10ug Ask for price

FMS, Active Human Recombinant Protein

GWB-149EA7 10ug Ask for price

CSK, Active Human Recombinant Protein

GWB-175A88 10ug Ask for price

CHK2, Active Human Recombinant Protein

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XIAP, Active Human Recombinant Protein

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Rheb, Active Human Recombinant Protein

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Her4, Active Human Recombinant Protein

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TRKB, Active Human Recombinant Protein

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DPP4, Active Human Recombinant Protein

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cRAF, Active Human Recombinant Protein

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MTAP, Active Human Recombinant Protein

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Flt1, Active Human Recombinant Protein

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DPP3, Active Human Recombinant Protein

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Akt1,Active Human Recombinant Protein

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Her2, Active Human Recombinant Protein

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Jak3, Active Human Recombinant Protein

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PLK3, Active Human Recombinant Protein

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ACK1, Active Human Recombinant Protein

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ROS1 Active Human Recombinant Protein

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NEK6, Active Human Recombinant Protein

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AHCY, Active Human Recombinant Protein

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p85a, Active Human Recombinant Protein

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PLK1, Active Human Recombinant Protein

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NEK2, Active Human Recombinant Protein

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aRAF, Active Human Recombinant Protein

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DPP8, Active Human Recombinant Protein

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MKK6 Active Human Recombinant Protein

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WWP1, Active Human Recombinant Protein

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DPP9, Active Human Recombinant Protein

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DPP7, Active Human Recombinant Protein

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Akt3, Active Human Recombinant Protein

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Flt3, Active Human Recombinant Protein

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TRKC, Active Human Recombinant Protein

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Aha1, Active Human Recombinant Protein

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Tie2, Active Human Recombinant Protein

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Tyk2 Active Human Recombinant Protein

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DDR2 Active Human Recombinant Protein

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MUSK, Active Human Recombinant Protein

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EGFR, Active Human Recombinant Protein

GWB-767301 10ug Ask for price

PDK1, Active Human Recombinant Protein

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MDM2 Active Human Recombinant Protein

GWB-1EA32E 20ug Ask for price

TRKA, Active Human Recombinant Protein

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Jak1 Active Human Recombinant Protein

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NEK7, Active Human Recombinant Protein

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USP7, Active Human Recombinant Protein

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NEK9, Active Human Recombinant Protein

GWB-PSA505 10ug Ask for price

TDP2, Active Human Recombinant Protein

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PrCP, Active Human Recombinant Protein

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cMet, Active Human Recombinant Protein

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PKM1, Active Human Recombinant Protein

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cKit, Active Human Recombinant Protein

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PYK2, Active Human Recombinant Protein

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PLK2, Active Human Recombinant Protein

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PDE1C, Active Human Recombinant Protein

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FGFR2, Active Human Recombinant Protein

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EphA2, Active Human Recombinant Protein

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PDE1A, Active Human Recombinant Protein

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PDE3A, Active Human Recombinant Protein

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IRAK4, Active Human Recombinant Protein

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USP14 Active Human Recombinant Protein

GWB-FADC72 50ug Ask for price

DAPK1 Active Human Recombinant Protein

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PDE7B, Active Human Recombinant Protein

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USP10 Active Human Recombinant Protein

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PDE3B, Active Human Recombinant Protein

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PDE1B, Active Human Recombinant Protein

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DYRK2 Active Human Recombinant Protein

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EphB2, Active Human Recombinant Protein

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STK33 Active Human Recombinant Protein

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EphB4, Active Human Recombinant Protein

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ZAP70, Active Human Recombinant Protein

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Hsp40, Active Human Recombinant Protein

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NEDD4, Active Human Recombinant Protein

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PDE7A, Active Human Recombinant Protein

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BRSK2, Active Human Recombinant Protein

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VPS34 Active Human Recombinant Protein

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UCHL3 Active Human Recombinant Protein

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Evaluating completely different sperm separation strategies for ART, via quantitative analysis of p53 protein

Context: Within the final 10 years, assisted reproductive applied sciences (ARTs) have supplied infertile {couples} a possibility to finish their reproductive undertaking. Nevertheless, the excessive failure price could possibly be defined with the complicated human replica system. In ART, the lower of the success is as a result of situations removed from the pure ones.
Goals: The intention of this research is to guage deoxyribonucleic acid (DNA) injury of spermatozoa earlier than and after choice procedures, utilizing a brand new approach capable of quantize sperm DNA injury.
Settings and design: They had been concerned 43 males domiciled completely in two areas with completely different Environmental Impression, HEI (excessive environmental impression) and LEI (Low environmental impression), they’re aged between 24 and 31 years with varied levels of dyspermia.
Topics and strategies: The 43 males had been divided into two teams: 21 in Group A (EIL) and 22 in Group B (EIH). The samples should be aliquoted into components of 0.5 mL: Group (a) Management, no processing; Group (b) Swim-up (SUP) from semen; Group (c) basic SUP; Group (d) density gradient centrifugation (DGC). All samples had been subjected to a quantitative dosage of p53 protein, earlier than and after processing.
Statistical evaluation used: For the event of the chance and significance of the information, the Scholar’s t-test was used.
Outcomes: From our information, it emerges that Teams D and B present a superior high quality about motility, vitality, and apoptosis indexes in comparison with different typical strategies. In Group B, apoptosis is akin to Group D, however they’ve barely decrease about motility and vitality.
Group C is the one which has decrease parameters than the opposite strategies. Concerning the analysis of p53 protein, the outcomes are conflicting with the analysis of apoptosis; in truth, in Group D, the values are considerably larger than the opposite strategies.
Conclusions: Sperm separation is a vital second in ART strategies. From our information, it emerges a better fragility of DNA within the male spermatozoa who reside completely in areas with excessive environmental impression.

SRPX (NM_006307) Human Over-expression Lysate

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EUR 628
Description: Transient overexpression lysate of sushi-repeat-containing protein, X-linked (SRPX), transcript variant 1

SRPX Over-expression Lysate Product

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Human SRPX2 Protein Lysate

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Human SRPX2 Protein Lysate 20ug

IHUSRPX2PLLY20UG each
EUR 211
Description: Human SRPX2 Protein Lysate 20ug

OCOA02119-20UG - SRPX2 Protein Lysate

OCOA02119-20UG 20ug
EUR 169

EP Reagent Biuret Reagent - 100ML

1011601 1L
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SRP72 Over-expression Lysate Product

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SRP19 Over-expression Lysate Product

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PEROXIDE BLOCKING REAGENT Reagent

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EP Reagent Iodoplatinate Reagent - 200ML

1046300 200ML
EUR 502.2

EP Reagent Molybdovanadic Reagent - 100ML

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EUR 59.4

SRP9 Over-expression Lysate Product

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EP Reagent Methoxyphenylacetic Reagent - 100ML

1053601 100ML
EUR 490.05

Protein expression

CRO-4-Ind each Ask for price

EP Reagent Phosphomolybdotungstic Reagent - 100ML

1065000 100ML
EUR 226.8

RFP expression Adenovirus, in vivo ready

AVP001-PBS 1x1011 IFU/ml x 50ul
EUR 455
Description: pre-made RFP expression adenovirus, provided in PBS solution.

CFP expression Adenovirus, in vivo ready

AVP002-PBS 1x1011 IFU/ml x 50ul
EUR 455
Description: pre-made CFP expression adenovirus, provided in PBS solution.

GFP expression Adenovirus, in vivo ready

AVP011-PBS 1x1011 IFU/ml x 50ul
EUR 455
Description: pre-made GFP expression adenovirus, provided in PBS solution.

YFP expression Adenovirus, in vivo ready

AVP012-PBS 1x1011 IFU/ml x 50ul
EUR 455
Description: pre-made YFP expression adenovirus, provided in PBS solution.

BFP expression Adenovirus, in vivo ready

AVP017-PBS 1x1011 IFU/ml x 50ul
EUR 455
Description: pre-made BFP expression adenovirus, provided in PBS solution.

SRP54 (NM_001146282) Human Over-expression Lysate

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EUR 395

SRP72 (NM_006947) Human Over-expression Lysate

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EUR 395

SRP68 (NM_014230) Human Over-expression Lysate

E45H09072-2 each
EUR 395

SRP54 (NM_003136) Human Over-expression Lysate

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SRP19 (NM_003135) Human Over-expression Lysate

E45H06529-2 each
EUR 395

SRP14 (NM_003134) Human Over-expression Lysate

E45H06530-2 each
EUR 395

SRP14 (NM_003134) Human Over-expression Lysate

LS006727 100ug
EUR 628
Description: Transient overexpression lysate of signal recognition particle 14kDa (homologous Alu RNA binding protein) (SRP14)

SRP19 (NM_003135) Human Over-expression Lysate

LS006728 100ug
EUR 628
Description: Transient overexpression lysate of signal recognition particle 19kDa (SRP19)

SRP54 (NM_003136) Human Over-expression Lysate

LS006729 100ug
EUR 628
Description: Transient overexpression lysate of signal recognition particle 54kDa (SRP54), transcript variant 1

SRP68 (NM_014230) Human Over-expression Lysate

LS006730 100ug
EUR 960
Description: Transient overexpression lysate of signal recognition particle 68kDa (SRP68)

SRP72 (NM_006947) Human Over-expression Lysate

LS006731 100ug
EUR 960
Description: Transient overexpression lysate of signal recognition particle 72kDa (SRP72)

SRP9 (NM_003133) Human Over-expression Lysate

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EUR 395

SRP9 (NM_003133) Human Over-expression Lysate

LS006726 100ug
EUR 628
Description: Transient overexpression lysate of signal recognition particle 9kDa (SRP9), transcript variant 2

AVIDIN/BIOTIN BLOCKING REAGENT Reagent

GWB-Q00289 30 ml Ask for price

Gordon-McLeod Reagent (Oxidase reagent)

R026-100ML 1 unit
EUR 7.76
Description: Gordon-McLeod Reagent (Oxidase reagent)

Oxidase Reagent (Gordon-Macleod Reagent)

76207 100 ml
EUR 3.38
Description: Part B

YLEX Expression Kit

FYY201-1KT 1 kit Ask for price

SRPRB Over-expression Lysate Product

GWB-C2D4F4 0.1 mg Ask for price

SRPK3 Over-expression Lysate Product

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SRPK2 Over-expression Lysate Product

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SRPK1 Over-expression Lysate Product

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EP Reagent Sulfomolybdic Reagent R3 - 1L

1086500 1L
EUR 398.25

TRIpure Reagent (same as Trizol Reagent)

MBS356001-INQUIRE INQUIRE Ask for price

h OCT4 expression Adenovirus, in vivo ready

AVP013-PBS 1x1011 IFU/ml x 50ul
EUR 455
Description: pre-made adenovirus express human stem cell factor, OCT4, provided in PBS solution for in vivo application.

h SOX2 expression Adenovirus, in vivo ready

AVP014-PBS 1x1011 IFU/ml x 50ul
EUR 455
Description: pre-made adenovirus express human stem cell factor, SOX2, provided in PBS solution for in vivo application.

293 Expression MAX-1

EXP-711-11 1ml
EUR 218
Description: 293 Expression MAX-1 is expressed from - -.

h LIN28 expression Adenovirus, in vivo ready

AVP015-PBS 1x1011 IFU/ml x 50ul
EUR 455
Description: pre-made adenovirus express human stem cell factor, LIN28, provided in PBS solution for in vivo application.

Total RNA Isolation Reagent (Reagent Based)

MBS402052-100mL 100mL
EUR 235

Total RNA Isolation Reagent (Reagent Based)

MBS402052-4Reactions 4Reactions
EUR 165

CNGA2 Expression vector

60642 20 µg
EUR 900
Description: The CNGA2 expression vector is designed to express human cyclic nucleotide-gated cation channel 2 (CNGA2) in mammalian cells.

CNGA2 Expression vector

GWB-PSFA61 20ug Ask for price

Genomic DNA Isolation Reagent (Reagent Based)

MBS402050-100mL 100mL
EUR 255

Genomic DNA Isolation Reagent (Reagent Based)

MBS402050-4Reactions 4Reactions
EUR 185

pYLEX1 - Expression Vector

FYY203-5MG 5mg Ask for price

LEXSYcon2.1 Expression Kit

EGE-1310ble 1Kit
EUR 979.85
Description: for constitutive cytosolic or secretory protein expression

LEXSYcon2.1 Expression Kit

EGE-1310bsd 1Kit
EUR 979.85
Description: for constitutive cytosolic or secretory protein expression

LEXSYcon2.1 Expression Kit

EGE-1310hyg 1Kit
EUR 979.85
Description: for constitutive cytosolic or secretory protein expression

LEXSYcon2.1 Expression Kit

EGE-1310neo 1Kit
EUR 979.85
Description: for constitutive cytosolic or secretory protein expression

LEXSYcon2.1 Expression Kit

EGE-1310pac 1Kit
EUR 979.85
Description: for constitutive cytosolic or secretory protein expression

LEXSYcon2.1 Expression Kit

EGE-1310sat 1Kit
EUR 979.85
Description: for constitutive cytosolic or secretory protein expression

Blunt E1 Expression Kit

abx098098-100l 100 µl
EUR 712.5

Blunt E1 Expression Kit

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Blunt E1 Expression Kit

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Blunt E2 Expression Kit

abx098873-100l 100 µl
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Blunt E2 Expression Kit

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Blunt E2 Expression Kit

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Blunt E2 Expression Kit

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RFP expression Adenovirus

AVP001 1x109 IFU/ml x 200ul
EUR 206.5
Description: pre-made RFP expression adenovirus, provided in DMEM medium.

CFP expression Adenovirus

AVP002 1x109 IFU/ml x 200ul
EUR 206.5
Description: pre-made CFP expression adenovirus, provided in DMEM medium.

GFP expression Adenovirus

AVP011 1x109 IFU/ml x 200ul
EUR 206.5
Description: pre-made GFP expression adenovirus, provided in DMEM medium.

YFP expression Adenovirus

AVP012 1x109 IFU/ml x 200ul
EUR 206.5
Description: pre-made YFP expression adenovirus, provided in DMEM medium.
Gregory

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