Potein Separation based on charge anisotropy by spherical polyelectrolyte brushes

choleratox

Selective protein separation based mostly on cost anisotropy by spherical polyelectrolyte brushes

 

Protein purification is of significant significance within the meals business, drug discovery and different associated fields. Amongst many separation strategies, polyelectrolyte (PE)-based section separation is developed and acknowledged as a low-cost purification approach. On this work, spherical polyelectrolyte brushes (SPBs) with excessive particular floor space was utilized to review the protein accessibility and selective protein binding on extremely charged nanoparticles (NPs) in addition to the selective section separation of proteins.
The correlation between cost anisotropy, protein binding, and section separation was investigated on numerous protein programs together with these proteins with comparable isoelectric factors (pI) similar to bovine serum albumin (BSA) and β-lactoglobulin (BLG), proteins with comparable molecular weights similar to BSA and hemoglobin (HB), and even protein variants (BLG-A and -B) with tiny distinction of amino acids.
The non-specific electrostatic interplay studied by turbidimetric titrations and isothermal calorimetry titration (ITC) signifies a particular binding between proteins and SPBs arising from the cost anisotropy of proteins.
An optimized output based mostly on selective protein binding on SPBs may very well be correlated for environment friendly protein separation by tuning exterior situations together with pH and ionic power. These findings due to this fact proved that section separation based mostly on selective protein adsorption by SPBs was an environment friendly various for proteins separation in contrast with conventional follow.

Reengineering anthrax toxin protecting antigen for improved receptor-specific protein supply

 

Background: To extend the scale of the druggable proteome, it will be extremely fascinating to plan environment friendly strategies to translocate designed binding proteins to the cytosol, as they might particularly goal flat and hydrophobic protein-protein interfaces.
If this may very well be carried out in a fashion depending on a cell floor receptor, two layers of specificity could be obtained: one for the cell sort and the opposite for the cytosolic goal. Bacterial protein toxins have naturally developed such programs.
Anthrax toxin consists of a pore-forming translocation unit (protecting antigen (PA)) and a separate protein payload. When engineering PA to ablate binding to its personal receptor and as an alternative binding to a receptor of alternative, by fusing a designed ankyrin repeat protein (DARPin), uptake in new cell varieties will be achieved.
Outcomes: Prepore-to-pore conversion of redirected PA already happens on the cell floor, limiting the quantity of PA that may be administered and thus limiting the quantity of delivered payload. We hypothesized that the reason being a scarcity of a stabilizing interplay with wild-type PA receptor.
We’ve got now reengineered PA to include the binding area of the anthrax receptor CMG2, adopted by a DARPin, binding to the receptor of alternative. This assemble is certainly stabilized, undergoes prepore-to-pore conversion solely in late endosomes, will be administered to a lot greater concentrations with out displaying toxicity, and consequently delivers a lot greater quantities of payload to the cytosol.
Conclusion: We imagine that this reengineered system is a vital step ahead to addressing environment friendly cell-specific supply of proteins to the cytosol.
Key phrases: Anthrax toxin; Cytosolic protein supply; DARPin; Protecting antigen.
choleratox

choleratox

Amylin Blocking Peptide

DF7715-BP 1mg
EUR 234

Amylin Blocking Peptide

20-abx062762
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  • 1 mg
  • 5 mg

Amylin (Feline)

017-01 500 μg
EUR 355.32

Amylin (14-24) (Human)

017-08 500 μg
EUR 64.8

Amylin (20-29) (Human)

017-09 500 μg
EUR 64.8

Amylin (17-37) (Human)

017-15 100 μg
EUR 140.4

Amylin (24-37) (Human)

017-16 100 μg
EUR 140.4

Amylin (20-29) (Feline)

017-02 200 μg
EUR 73.44

Amylin (8-37) (Rat)

017-14 200 μg
EUR 214.92

Amylin (1-13) (Human)

017-06 200 μg
EUR 108

Amylin (Rat, Mouse)

017-11 500 μg
EUR 355.32

Amylin / IAPP (Human)

017-03 100 μg
EUR 140.4

Amylin amide (Human)

017-04 100 μg
EUR 114.48

Amylin (25-37) amide (Human)

017-10 500 μg
EUR 86.4

[Tyr0]-Amylin (1-13) (Human)

017-07 100 μg
EUR 355.32

Anantin (linear sequence) Peptide

abx266971-1ml 1 ml
EUR 762.5

Anantin (linear sequence) Peptide

abx266971-200l 200 µl
EUR 325

Amylin (Feline) - Antibody

H-017-01 50 μl
EUR 238.68

LL 37, reverse sequence Peptide

abx267003-1ml 1 ml
EUR 975

LL 37, reverse sequence Peptide

abx267003-200l 200 µl
EUR 400

Amylin (1-13) (Human) - Antibody

H-017-06 100 μl
EUR 295.92

Amylin (Feline) - RIA Kit

RK-017-01 125 tubes
EUR 980.64

Amylin (1-13) (Human) - EIA Kit

EK-017-06 96 wells
EUR 603.72

Amylin (1-13) (Human) - RIA Kit

RK-017-06 125 tubes
EUR 980.64

Amylin (21-27) / IAPP(21-27) (Human)

017-20 500 μg
EUR 206.28

Amylin (28-33) / IAPP(28-33) (Human)

017-22 500 μg
EUR 206.28

Amylin (Rat, Mouse) - Antibody

H-017-11 50 μl
EUR 238.68

Amylin / IAPP (Human) - Antibody

H-017-03 50 μl
EUR 238.68

Amylin (25-37) amide (Human) - RIA Kit

RK-017-10 125 tubes
EUR 980.64

Erythropoietin Mimetic Peptide Sequence 20

H-4344.0001 1.0mg
EUR 544.8
Description: Sum Formula: C72H99N17O17S2; CAS# [203397-62-0] net

Erythropoietin Mimetic Peptide Sequence 20

H-4344.0005 5.0mg
EUR 2067.6
Description: Sum Formula: C72H99N17O17S2; CAS# [203397-62-0] net

Amylin amide (Human) - Antibody

H-017-04 100 μl
EUR 469.8

Amylin (Rat, Mouse) - EIA Kit

EK-017-11 96 wells
EUR 603.72

Amylin (Rat, Mouse) - RIA Kit

RK-017-11 125 tubes
EUR 980.64

Amylin / IAPP (Human) - EIA Kit

EK-017-03 96 wells
EUR 603.72

Substance P reversed sequence Peptide

20-abx266314
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Substance P reversed sequence Peptide

abx266314-100l 100 µl
EUR 312.5

Substance P reversed sequence Peptide

abx266314-1ml 1 ml
EUR 812.5

Substance P reversed sequence Peptide

abx266314-200l 200 µl
EUR 462.5

Rabbit anti Amylin Peptide Polyclonal Antibody

TA354264 100 µg Ask for price

Amylin amide (Human) - RIA Kit

RK-017-04 125 tubes
EUR 980.64

Amylin (Rat, Mouse) - FAM Labeled

FG-017-11A 1 nmol
EUR 267.84

Amylin / IAPP (Human) - FAM Labeled

FG-017-03A 1 nmol
EUR 267.84

Amylin (Feline) - I-125 Labeled

T-017-01 10 μCi
EUR 1145.88

Amylin (1-13) (Human) - Fluorescent EIA Kit

FEK-017-06 96 wells
EUR 660.96

Amylin (25-37) amide (Human) - I-125 Labeled

T-017-10 10 μCi
EUR 1145.88

Amylin (1-13) (Human) - Purified IgG Antibody

G-017-06 100 μg
EUR 336.96

[Tyr0]-Amylin (1-13) (Human) - I-125 Labeled

T-017-07 10 μCi
EUR 1145.88

Amylin (Feline) - Purified IgG Antibody

G-017-01 400 μg
EUR 506.52

Rat Amylin Control/blocking peptide # 3

AMYL13-P 100 ug
EUR 196.8

Amylin (Rat, Mouse) - I-125 Labeled

T-017-11 10 μCi
EUR 1145.88

Amylin / IAPP (Human) - I-125 Labeled

T-017-03 10 μCi
EUR 1145.88

Human Amylin Control/blocking peptide # 2

AMYL12-P 100 ug
EUR 196.8

Amylin (Rat, Mouse) - Fluorescent EIA Kit

FEK-017-11 96 wells
EUR 660.96

Amylin (Rat, Mouse) - Purified IgG Antibody

G-017-11 400 μg
EUR 506.52

Amylin / IAPP (Human) - Purified IgG Antibody

G-017-03 100 μg
EUR 469.8

Amylin (23-37) Var. F23R / IAPP (23-37) Var. F23R (Human)

017-18 100 μg
EUR 140.4

Amylin (1-13) (Human) - I-125 Labeled Purified IgG

T-G-017-06 10 μCi
EUR 1145.88

Amylin amide (Human) - Purified IgG Antibody

G-017-04 100 μg
EUR 469.8

Amylin (25-37) amide (Human) - I-125 Labeled Purified IgG

T-G-017-10 10 μCi
EUR 1145.88

Human Amylin (1-37 aa) Control/blocking peptide #1

AMYL11-P 100 ug
EUR 196.8

Amylin (Feline) - I-125 Labeled Purified IgG

T-G-017-01 10 μCi
EUR 1145.88

Amylin amide (Human) - FAM Labeled Purified IgG

FG-G-017-04A 100 μl
EUR 604.8

Amylin amide (Human) - FITC Labeled Purified IgG

FG-G-017-04B 100 μl
EUR 604.8

Amylin / IAPP (Human) - EIA Kit, CE Mark Certified

EK-017-03CE 96 wells
EUR 629.64

Amylin amide (Human) - Biotin Labeled Purified IgG

B-G-017-04 100 μl
EUR 604.8

Amylin (Rat, Mouse) - I-125 Labeled Purified IgG

T-G-017-11 10 μCi
EUR 1145.88

Amylin / IAPP (Human) - I-125 Labeled Purified IgG

T-G-017-03 10 μCi
EUR 1145.88

Borrelia Vmp-like sequence expressed (VlsE) C6 peptide (>90%) for ELISA

VLSE65-UL 100 ug
EUR 416.4

Fpr (untagged) - Rat formyl peptide receptor, related sequence 3 (Fpr-rs3)

RN216422 10 µg Ask for price

Mouse Formyl peptide receptor-related sequence 1 (FPR-S1) ELISA Kit

abx524265-96tests 96 tests
EUR 687.5

Mouse Formyl peptide receptor-related sequence 3 (FPR-RS3) ELISA Kit

abx524266-96tests 96 tests
EUR 687.5

Mouse Formyl peptide receptor-related sequence 4 (FPR-RS4) ELISA Kit

abx524267-96tests 96 tests
EUR 687.5

Mouse Formyl peptide receptor-related sequence 6 (FPR-RS6) ELISA Kit

abx524268-96tests 96 tests
EUR 687.5

Mouse Formyl peptide receptor-related sequence 7 (FPR-RS7) ELISA Kit

abx524269-96tests 96 tests
EUR 687.5

Borrelia Vmp-like sequence expressed (VlsE) C6 peptide-Biotin (>90%) for ELISA

VLSE65-BTN 100 ug
EUR 489.6

Fpr (untagged) - Mouse formyl peptide receptor, related sequence 3 (Fpr-rs3), (10ug)

MC208504 10 µg Ask for price

Fpr (untagged) - Mouse formyl peptide receptor, related sequence 4 (Fpr-rs4), (10ug)

MC208505 10 µg Ask for price

Fpr (untagged) - Mouse formyl peptide receptor, related sequence 6 (Fpr-rs6), (10ug)

MC214494 10 µg Ask for price

DiagSupport™ Amine PEG-Polystyrene Resin, 90 µm, 0.18-0.22 mmol/g, Long Peptide Sequence

SPS-RA23-020 1 g
EUR 660

DiagSupport™ Bromo PEG-Polystyrene Resin, 90 µm, 0.18-0.22 mmol/g, Long Peptide Sequence

SPS-RA23-063 1 g
EUR 660

Impact of methotrexate therapy on the expression of epidermal-fatty acid-binding protein (E-FABP) and apolipoproteins in sufferers with psoriasis

Introduction: Epidermal-fatty acid-binding protein (E-FABP) is a marker of transiently amplifying cells that are fashioned from stem cells in dermis. Their position is an uptake of fatty acids and metabolism. Psoriatic keratinocytes overexpress E-FABPs, which ends up in acanthosis and will clarify the lipid’s disturbances in psoriasis.
Purpose: Evaluation of FABP and apolipoprotein expression in sufferers handled with methotrexate (MTX).
Materials and strategies: FABP expression within the lesional and perilesional psoriatic pores and skin from 11 male sufferers in comparison with 5 wholesome pores and skin samples had been evaluated by immunohistochemistry. FABP, apolipoprotein A1 (ApoA1) and B (ApoB) serum ranges had been assessed by ELISA. These parameters had been evaluated earlier than and after therapy with subcutaneous MTX (15 mg/wk for 12 weeks).
Outcomes: Expression of E-FABP was decrease within the management group than within the lesional and perilesional psoriatic pores and skin, earlier than and after therapy. After therapy the expression decreased within the lesional and perilesional pores and skin. Serum E-FABP was greater within the management group (482.855 ±240.550 pg/ml) in comparison with sufferers, however not statistically considerably.
After MTX therapy, a statistically important discount was noticed in psoriatic sufferers. ApoA1 ranges didn’t differ within the management and sufferers teams, each earlier than and after therapy. In distinction, ApoB ranges didn’t differ statistically between the management group (1447.126 ±311.11 ng/ml) and sufferers earlier than therapy, whereas they had been the bottom after therapy (1081.67 ±117.83 ng/ml vs. 808.306 ±103.72 ng/ml; p < 0.01).
Conclusions: Our examine confirms the useful impact of MTX, not solely as an anti-proliferative impact, but in addition lowering the cardiovascular danger by lowering atherogenic ApoB.
Key phrases: apolipoprotein; fatty acid-binding protein; methotrexate; psoriasis.

Nucleolar Protein 3 (NOL3) Antibody

20-abx004831
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Nucleolar Protein 3 (NOL3) Antibody

abx004831-100l 100 µl
EUR 400

Nucleolar Protein 3 (NOL3) Antibody

abx004831-20l 20 µl
EUR 175

Nucleolar Protein 3 (NOL3) Antibody

abx004831-50l 50 µl
EUR 275

Nucleolar Protein 3 (NOL3) Antibody

abx013023-100g 100 µg
EUR 237.5

Nucleolar Protein 3 (NOL3) Antibody

abx013023-10g 10 µg
EUR 43.75

Nucleolar Protein 3 (NOL3) Antibody

abx013023-200g 200 µg
EUR 312.5

Nucleolar Protein 3 (NOL3) Antibody

abx235784-100g 100 µg
EUR 350

Nucleolar Protein 3 (NOL3) Antibody

abx037829-96tests 96 tests
EUR 337.5

Nucleolar Protein 3 (NOL3) Antibody

abx038406-100g 100 µg
EUR 337.5

Nucleolar Protein 3 (NOL3) Antibody

abx114218-100l 100 µl
EUR 612.5

Nucleolar Protein 3 (NOL3) Antibody

abx210877-100l 100 µl
EUR 350

Nucleolar Protein 3 (NOL3) Antibody

abx210877-50l 50 µl
EUR 250

Nucleolar Protein 3 (NOL3) Antibody

abx320690-100l 100 µl
EUR 350

Nucleolar Protein 3 (NOL3) Antibody

abx320690-50l 50 µl
EUR 250

Nucleolar Protein 3 (NOL3) Antibody

abx324845-100g 100 µg
EUR 250

Nucleolar Protein 3 (NOL3) Antibody

abx324845-50g 50 µg
EUR 187.5

Nucleolar Protein 3 (NOL3) Antibody

abx318527-100l 100 µl
EUR 250

Nucleolar Protein 3 (NOL3) Antibody

abx318527-50l 50 µl
EUR 162.5

Nucleolar Protein 3 (NOL3) Antibody (HRP)

20-abx304651
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  • 100 ug
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Nucleolar Protein 3 (NOL3) Antibody (HRP)

abx304651-100g 100 µg
EUR 362.5

Nucleolar Protein 3 (NOL3) Antibody (HRP)

abx304651-20g 20 µg
EUR 162.5

Nucleolar Protein 3 (NOL3) Antibody (HRP)

abx304651-50g 50 µg
EUR 250

Nucleolar Protein 3 (NOL3) Antibody (FITC)

20-abx304652
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  • 100 ug
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Nucleolar Protein 3 (NOL3) Antibody (FITC)

abx304652-100g 100 µg
EUR 362.5

Nucleolar Protein 3 (NOL3) Antibody (FITC)

abx304652-20g 20 µg
EUR 162.5

Nucleolar Protein 3 (NOL3) Antibody (FITC)

abx304652-50g 50 µg
EUR 250

Nucleolar Protein 3 (NOL3) Antibody (Biotin)

20-abx304653
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  • 100 ug
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Nucleolar Protein 3 (NOL3) Antibody (Biotin)

abx304653-100g 100 µg
EUR 362.5

Nucleolar Protein 3 (NOL3) Antibody (Biotin)

abx304653-20g 20 µg
EUR 162.5

Nucleolar Protein 3 (NOL3) Antibody (Biotin)

abx304653-50g 50 µg
EUR 250

Nucleolar protein 58 Antibody (HRP)

20-abx108572
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  • 100 ug
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Nucleolar protein 58 Antibody (FITC)

20-abx107151
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Nucleolar protein 58 Antibody (Biotin)

20-abx105733
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NOP2 Antibody / Nucleolar protein 2

F53988-0.05ML 0.05 ml
EUR 140.25
Description: Nucleolar protein 2 is an S-adenosyl-L-methionine-dependent methyltransferase that specifically methylates the C(5) position of cytosine 4447 in 28S rRNA (Probable). May play a role in the regulation of the cell cycle and the increased nucleolar activity that is associated with the cell proliferation.

NOP2 Antibody / Nucleolar protein 2

F53988-0.2ML 0.2 ml
EUR 322.15
Description: Nucleolar protein 2 is an S-adenosyl-L-methionine-dependent methyltransferase that specifically methylates the C(5) position of cytosine 4447 in 28S rRNA (Probable). May play a role in the regulation of the cell cycle and the increased nucleolar activity that is associated with the cell proliferation.

Nucleolar Protein 8 (NOL8) Antibody

20-abx324504
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  • 100 ug
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Nucleolar Protein 6 (NOL6) Antibody

20-abx301690
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Nucleolar Protein 9 (NOL9) Antibody

abx034569-400ul 400 ul
EUR 627.6

Nucleolar Protein 9 (NOL9) Antibody

abx034569-80l 80 µl
EUR 343.2

Nucleolar Protein 9 (NOL9) Antibody

20-abx211330
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Nucleolar Protein 9 (NOL9) Antibody

20-abx124786
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Nucleolar Protein 4 (NOL4) Antibody

abx235785-100ug 100 ug
EUR 577.2

Nucleolar Protein 6 (NOL6) Antibody

abx235786-100ug 100 ug
EUR 577.2

Nucleolar Protein 6 (NOL6) Antibody

abx235787-100ug 100 ug
EUR 610.8

Nucleolar Protein 8 (NOL8) Antibody

abx235788-100ug 100 ug
EUR 610.8

Nucleolar Protein 9 (NOL9) Antibody

abx235789-100ug 100 ug
EUR 661.2

Nucleolar Protein 4 (NOL4) Antibody

20-abx217203
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  • 100 ug
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Nucleolar Protein 8 (NOL8) Antibody

20-abx217204
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  • 100 ug
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Nucleolar Protein 7 (NOL7) Antibody

20-abx217284
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  • 100 ug
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Nucleolar Protein 8 (NOL8) Antibody

20-abx141132
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Nucleolar Protein 9 (NOL9) Antibody

abx122121-100ug 100 ug
EUR 469.2

Nucleolar Protein 8 (NOL8) Antibody

20-abx114219
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  • 150 ul
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Nucleolar Protein 4 (NOL4) Antibody

abx029414-400ul 400 ul
EUR 627.6

Nucleolar Protein 4 (NOL4) Antibody

abx029414-80l 80 µl
EUR 343.2

Nucleolar Protein 4 (NOL4) Antibody

abx235785-100g 100 µg
EUR 350

Nucleolar Protein 6 (NOL6) Antibody

abx235786-100g 100 µg
EUR 350

Nucleolar Protein 6 (NOL6) Antibody

abx235787-100g 100 µg
EUR 350

Nucleolar Protein 8 (NOL8) Antibody

abx235788-100g 100 µg
EUR 350

Nucleolar Protein 9 (NOL9) Antibody

abx235789-100g 100 µg
EUR 350

Nucleolar Protein 6 (NOL6) Antibody

abx301690-100g 100 µg
EUR 362.5

Nucleolar Protein 6 (NOL6) Antibody

abx301690-20g 20 µg
EUR 162.5

Nucleolar Protein 6 (NOL6) Antibody

abx301690-50g 50 µg
EUR 250

Nucleolar Protein 9 (NOL9) Antibody

abx122121-100l 100 µl Ask for price

Nucleolar Protein 9 (NOL9) Antibody

abx122121-20l 20 µl
EUR 337.5

Nucleolar Protein 9 (NOL9) Antibody

abx122121-50l 50 µl Ask for price

Nucleolar Protein 8 (NOL8) Antibody

abx114219-100l 100 µl
EUR 612.5

Nucleolar Protein 9 (NOL9) Antibody

abx034569-100g 100 µg
EUR 281.25

Nucleolar Protein 7 (NOL7) Antibody

abx217284-100tests 100 tests
EUR 387.5

Nucleolar Protein 7 (NOL7) Antibody

abx217284-200tests 200 tests Ask for price

Nucleolar Protein 7 (NOL7) Antibody

abx217284-20tests 20 tests
EUR 300

Nucleolar Protein 4 (NOL4) Antibody

abx029414-400l 400 µl
EUR 518.75

Nucleolar Protein 9 (NOL9) Antibody

abx211330-100l 100 µl
EUR 350

Nucleolar Protein 9 (NOL9) Antibody

abx211330-50l 50 µl
EUR 250

Nucleolar Protein 4 (NOL4) Antibody

abx375641-96tests 96 tests
EUR 337.5

Nucleolar Protein 7 (NOL7) Antibody

abx375642-96tests 96 tests
EUR 337.5

Nucleolar Protein 8 (NOL8) Antibody

abx324504-100g 100 µg
EUR 250

Nucleolar Protein 8 (NOL8) Antibody

abx324504-50g 50 µg
EUR 187.5

Nucleolar Protein 10 (NOL10) Antibody

20-abx324613
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Nucleolar Protein 10 (NOL10) Antibody

20-abx302728
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  • 100 ug
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Nucleolar Protein 12 (NOL12) Antibody

abx235783-100ug 100 ug
EUR 610.8

Nucleolar Protein 11 (NOL11) Antibody

20-abx217281
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  • 100 ug
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Nucleolar Protein 12 (NOL12) Antibody

20-abx217282
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  • 100 ug
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Nucleolar Protein 14 (NOL14) Antibody

20-abx217283
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  • 100 ug
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Gregory

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